Bureau M, Brun-Pascaud M, Colas-Linhart N, Vivet P, Pocidalo J J
Bull Eur Physiopathol Respir. 1983 May-Jun;19(3):279-84.
Vascular inflammatory response to hyperoxia (FIO2 greater than 0.98) was studied in awake jugular and carotid catheterized rats. Simultaneous i.v. injection of 125I albumin (125I A), 99mTc polymorphonuclear cells (99mTc PMN) and 51Cr red blood cells (51Cr RBC) allowed to study both the macromolecule exchange through vascular endothelium and the leukocyte uptake by several organs (liver, lungs, spleen) with respect to radiolabelled red blood cells, as an intravascular reference. Rats were exposed to O2 for 24, 38 and 45 h. They were anesthetized and killed by exanguination 15 to 120 min following the tracer injection. After 45 h exposure, the plasmatic 125I A half-life decreased significantly (158 +/- 42 min for the control; 106 +/- 34 min for the exposed animals). The ratio 125I A/51Cr RBC varied significantly in the lung. The iodinated albumin exchange through lung vascular endothelium was altered at the 24th h, with a significant difference reached by the 45th h. At the same time, the pulmonary decreasing curve of 99mTc/51Cr RBC ratio versus time was not not modified. In our experimental conditions, there was no detectable variation in the lung uptake and vascular transit of PMN cells. The discussion of the results must be related with the technics used in the present work when the albumin exchange increased.
在清醒状态下经颈静脉和颈动脉插管的大鼠中研究了对高氧（FIO2大于0.98）的血管炎症反应。同时静脉注射125I白蛋白（125I A）、99mTc多形核细胞（99mTc PMN）和51Cr红细胞（51Cr RBC），以便相对于放射性标记的红细胞作为血管内参考，研究大分子通过血管内皮的交换以及几种器官（肝脏、肺、脾脏）对白细胞的摄取。将大鼠暴露于氧气中24、38和45小时。在注射示踪剂后15至120分钟，将它们麻醉并放血处死。暴露45小时后，血浆125I A半衰期显著降低（对照组为158±42分钟；暴露组动物为106±34分钟）。肺中125I A/51Cr RBC的比值有显著变化。经肺血管内皮的碘化白蛋白交换在第24小时发生改变，到第45小时达到显著差异。同时，99mTc/51Cr RBC比值相对于时间的肺下降曲线未改变。在我们的实验条件下，PMN细胞的肺摄取和血管转运没有可检测到的变化。当白蛋白交换增加时，结果的讨论必须与本研究中使用的技术相关。
