Silver J C, Andrews D R, Pekkala D
Can J Biochem Cell Biol. 1983 Jun;61(6):447-55. doi: 10.1139/o83-060.
Heat shock induced by an increase in temperature from 28 to 37 degrees C led to changes in synthesis and phosphorylation of cytoplasmic and nuclear proteins in the aquatic fungus Achlya. In the cytoplasmic fraction a marked increase in [35S]methionine labelling of proteins in the molecular weight range of 96 000, 85 000, 74 000, and 70 000 was observed. Two-dimensional electrophoresis resolved each of these classes of proteins into several components. Major changes in the nuclear fraction included the increased [35S]methionine labelling of 43 000 and 28 000-23 000 proteins. A marked decline in the synthesis of many other proteins was also evident. The heat-shock-induced changes in labelling patterns became evident as early as 20 to 60 min after treatment, but they were transient. With continued incubation at the heat-shock temperature, the cells appeared to adapt to the new temperature conditions. Both cytoplasmic and nuclear proteins returned to nearly normal labelling patterns within 100 to 140 min at 37 degrees C. Changes in phosphorylation of histone and nonhistone nuclear proteins were also noted. Achlya histone H3 and the putative oomycete-specific histone "alpha" appeared highly phosphorylated after heat shock. Since phosphorylation of histone H3 is primarily associated with chromatin condensation, it is possible that rapid chromatin condensation is an initial response to heat shock in Achlya.
温度从28摄氏度升至37摄氏度所引发的热休克，导致水生真菌绵霉中细胞质和细胞核蛋白质的合成与磷酸化发生变化。在细胞质部分，观察到分子量范围为96000、85000、74000和70000的蛋白质的[35S]甲硫氨酸标记显著增加。二维电泳将这些蛋白质类别中的每一种都解析为几个组分。细胞核部分的主要变化包括43000以及28000 - 23000蛋白质的[35S]甲硫氨酸标记增加。许多其他蛋白质的合成也明显下降。热休克诱导的标记模式变化早在处理后20至60分钟就变得明显，但它们是短暂的。在热休克温度下持续孵育时，细胞似乎适应了新的温度条件。在37摄氏度下，细胞质和细胞核蛋白质在100至140分钟内恢复到几乎正常的标记模式。还注意到组蛋白和细胞核非组蛋白磷酸化的变化。热休克后，绵霉组蛋白H3和假定的卵菌特异性组蛋白“α”似乎高度磷酸化。由于组蛋白H3的磷酸化主要与染色质凝聚相关，因此快速染色质凝聚可能是绵霉对热休克的初始反应。
