Gwynne D I, Brandhorst B P
J Bacteriol. 1982 Feb;149(2):488-93. doi: 10.1128/jb.149.2.488-493.1982.
When exponentially growing cultures of Achlya ambisexualis strain E87 were raised from their normal growth temperature of 30 degrees C to 35 degrees C, the rates of synthesis of a small number of proteins were dramatically increased. The most predominant proteins synthesized in response to heat shock had molecular weights of 70,000 and 78,000, and their increased synthesis was detected as early as 10 min after the shift to 35 degrees C. Changes in the populations of translatable messenger RNAs during heat shock showed that the levels of the mRNA's for all the major induced proteins correlated very closely with the alterations in the in vivo patterns, suggesting a transcriptional level of control of their synthesis. When after a period of heat shock (60 min) the cultures were shifted back to 30 degrees C, recovery of the preshock patterns of protein synthesis was attained after several hours. Different proteins show temporally distinct patterns of recovery. During recovery the levels of translatable mRNA's for the induced proteins also correlated closely with the patterns of in vivo protein synthesis.
当两性绵霉E87菌株的指数生长培养物从其正常生长温度30℃升高到35℃时，少数蛋白质的合成速率显著增加。响应热休克合成的最主要蛋白质的分子量为70000和78000，早在转移到35℃后10分钟就检测到它们合成增加。热休克期间可翻译信使RNA群体的变化表明，所有主要诱导蛋白的mRNA水平与体内模式的变化非常密切相关，这表明其合成受转录水平的控制。当热休克一段时间（60分钟）后，培养物转回30℃，数小时后蛋白质合成恢复到休克前模式。不同蛋白质显示出时间上不同的恢复模式。恢复期间，诱导蛋白的可翻译mRNA水平也与体内蛋白质合成模式密切相关。
