Gunduz N, Zheng S, Fisher B
Cancer. 1983 Oct 1;52(7):1251-6. doi: 10.1002/1097-0142(19831001)52:7<1251::aid-cncr2820520720>3.0.co;2-r.
Despite widespread use there are disadvantages associated with biochemical methods for ER determination. Since there is increasing emphasis on earlier detection of primary breast cancers there is clearly a need for a method which requires only small numbers of tumor cells. A variety of other clinical situations exist in which the availability of a technic that could supply information regarding tumor ER from a small cell population would be helpful. This report presents our experience with 17-fluoresceinated estrone (17-FE) binding for detecting the proportion of estrone binding cells in samples obtained by fine-needle aspiration of tumors prior to their removal. The findings from the aspirated cells employing 17-FE were compared with results obtained from aliquots of the same tumors following their removal using both cytochemical (17-FE) and biochemical (3H-estradiol binding) methods for ER determination. When biochemically determined ER values (fmol/mg cytosol protein) were compared to the proportion of fluorescent cells observed either in cells from needle aspirates prior to tumor removal or in cells from the removed tumor there was no direct quantitative relationship. That lack of correlation is to be expected and does not indicate that one or the other method is inappropriate. It was found, however, that 100% of tumors having ER levels greater than or equal to 10 fmol had greater than or equal to 10% marker positive cells. Fifty-four percent of tumors with a negative or borderline (less than 10 fmol) ER level had less than 10% marker positive cells. The less satisfactory correlation in biochemically ER negative tumors may be related to false negative biochemical determinations. The better correlation between results from the biochemical method and those with 17-FE binding using needle aspirates than between the former and the latter when cells from fragments of removed tumor were used for cytochemical analysis, and the lack of concordance in 17-FE binding by the two cell suspensions (from needle aspirates and from parts of the removed tumor) may be related to the heterogeneity of the cellular composition of a tumor as regards ER content and consequently to the methods of sampling. The authors' experience in this and previous studies using 17-FE for determination of the proportion of putatively ER positive cells indicate that the method is worthy of further evaluation and consideration for experimental and clinical use.
尽管生化方法在雌激素受体（ER）测定中被广泛应用，但仍存在一些缺点。由于越来越强调早期发现原发性乳腺癌，显然需要一种仅需少量肿瘤细胞的方法。还存在多种其他临床情况，在这些情况下，一种能够从小细胞群体中提供有关肿瘤ER信息的技术将很有帮助。本报告介绍了我们使用17-荧光雌酮（17-FE）结合法检测肿瘤切除前通过细针穿刺获得的样本中雌酮结合细胞比例的经验。将穿刺细胞采用17-FE的检测结果与同一肿瘤切除后使用细胞化学法（17-FE）和生化法（3H-雌二醇结合法）测定ER的等分样本结果进行比较。当将生化测定的ER值（fmol/mg胞浆蛋白）与在肿瘤切除前针吸细胞或切除肿瘤细胞中观察到的荧光细胞比例进行比较时，不存在直接的定量关系。这种缺乏相关性是可以预料的，并不表明这两种方法中的任何一种不合适。然而，发现ER水平大于或等于10 fmol的肿瘤中有100%的标记阳性细胞大于或等于10%。ER水平为阴性或临界值（小于10 fmol）的肿瘤中有54%的标记阳性细胞小于10%。生化ER阴性肿瘤中相关性较差可能与生化测定的假阴性有关。与使用切除肿瘤碎片细胞进行细胞化学分析时相比，生化方法与针吸样本中17-FE结合结果之间的相关性更好，而两种细胞悬液（针吸样本和切除肿瘤部分）的17-FE结合缺乏一致性可能与肿瘤细胞组成在ER含量方面的异质性有关，因此也与采样方法有关。作者在本次及之前使用17-FE测定推定ER阳性细胞比例的研究中的经验表明，该方法值得进一步评估并考虑用于实验和临床。
