Hamilton E, Dobbin J
Cell Tissue Kinet. 1983 Sep;16(5):473-81.
Cell kinetic parameters measured by the percentage labelled mitoses (PLM) technique have been compared with those derived from continuous labelling and stathmokinetic data in the mouse tumour Carcinoma NT. In [3H]TdR labelled tumours, a PLM curve showed the length of DNA synthesis (TS) to be 9 hr. With continuous labelling, 2.5% of cells entered S phase per hour and TS was 16 hr. Six per cent of cells incorporated [3H]TdR between 1 and 3 hr after an injection of the label. The stathmokinetic technique showed that the rate of entry to mitosis was 2.0% hr. The turnover time (TT) of the population was found to be 16 hr with the PLM data and 35 and 37 hr with the continuous labelling and stathmokinetic data, respectively. The PLM technique therefore showed the mean cell cycle parameters to be about half the values obtained with other methods of measurement. We conclude that in Carcinoma NT there is a wide range of cell cycle times. The continuous labelling and stathmokinetic data represent the mean cell cycle parameters, while the PLM data give the minimum values. A cell loss factor calculated from the PLM data would, therefore, be too high by a factor of two.
通过标记有丝分裂百分数（PLM）技术测得的细胞动力学参数，已与从小鼠肿瘤NT癌的连续标记和静止动力学数据得出的参数进行了比较。在[3H]TdR标记的肿瘤中，一条PLM曲线显示DNA合成时长（TS）为9小时。采用连续标记时，每小时有2.5%的细胞进入S期，TS为16小时。注射标记物后1至3小时内，6%的细胞掺入了[3H]TdR。静止动力学技术表明，进入有丝分裂的速率为每小时2.0%。根据PLM数据，群体周转时间（TT）为16小时，而根据连续标记和静止动力学数据，TT分别为35小时和37小时。因此，PLM技术显示的平均细胞周期参数约为用其他测量方法所得值的一半。我们得出结论，在NT癌中存在广泛的细胞周期时间范围。连续标记和静止动力学数据代表平均细胞周期参数，而PLM数据给出的是最小值。因此，根据PLM数据计算出的细胞丢失因子会高出两倍。
