Hamilton E, Dobbin J
Cell Tissue Kinet. 1983 Sep;16(5):483-92.
Cell cycle parameters measured by the percentage labelled mitoses (PLM) technique have been compared with the results of grain counting and continuous labelling studies in the mouse tumour Carcinoma NT. In [3H]deoxyuridine( [3H]UdR)-labelled tumours a PLM curve showed that the mean length of DNA synthesis (TS) was 7 to 9 hr and the mean cell cycle time (TC) was 16 hr. The turnover time (TT) was between 13 and 17 hr. In contrast, the rate of grain count halving showed that mean TC was 40 hr and TS was at least 19 hr. Cells entered S phase at a rate of 2.3%/hr with continuous labelling, which gave values of 22 hr for TS and 39 to 44 hr for TT. The PLM data therefore gave mean kinetic parameters which were less than half the lengths shown by the other techniques. We conclude that in Carcinoma NT there is a wide range of cell cycle times. The grain count halving and continuous labelling data showed the true mean values of TS and TC or TT, while the PLM data showed only the fastest cycle times. Therefore in this tumour, calculating the cell loss factor from PLM data would give a value too high by at least a factor of two.
通过标记有丝分裂百分数（PLM）技术测量的细胞周期参数，已与小鼠肿瘤NT癌的颗粒计数和连续标记研究结果进行了比较。在[3H]脱氧尿苷（[3H]UdR）标记的肿瘤中，一条PLM曲线显示DNA合成的平均时长（TS）为7至9小时，平均细胞周期时长（TC）为16小时。周转时间（TT）在13至17小时之间。相比之下，颗粒计数减半率显示平均TC为40小时，TS至少为19小时。连续标记时，细胞以2.3%/小时的速率进入S期，得出TS值为22小时，TT值为39至44小时。因此，PLM数据得出的平均动力学参数不到其他技术所显示时长的一半。我们得出结论，在NT癌中存在广泛的细胞周期时长范围。颗粒计数减半和连续标记数据显示了TS以及TC或TT的真实平均值，而PLM数据仅显示了最快的周期时长。因此，在这种肿瘤中，根据PLM数据计算细胞丢失因子会得出至少高出两倍的值。
