Saito Z, Mimoh N, Hifumi S, Takeda R
Clin Chim Acta. 1983 Jul 15;131(3):239-46. doi: 10.1016/0009-8981(83)90093-1.
High pressure liquid chromatography (HPLC) was demonstrated to be a good tool for the separation, identification and quantitation of corticosteroids (CS) extracted from homogenized tissue of adrenal glands and adrenal tumors in patients with hypercorticism. A chromatographic system consisting of Sorbax-SIL and Sorbax-CN columns, organic solvent extraction and a UV detector was used to analyze both more polar and less polar corticosteroids in the adrenal tissue.
高压液相色谱法(HPLC)被证明是一种用于分离、鉴定和定量从肾上腺皮质功能亢进患者的肾上腺和肾上腺肿瘤匀浆组织中提取的皮质类固醇(CS)的良好工具。使用由Sorbax-SIL柱和Sorbax-CN柱、有机溶剂萃取和紫外检测器组成的色谱系统来分析肾上腺组织中极性较强和较弱的皮质类固醇。