Ichikawa Y, Hiwatashi A, Nishii Y
Comp Biochem Physiol B. 1983;75(3):479-88. doi: 10.1016/0305-0491(83)90362-0.
Cholecalciferol (vitamin D3) 25-hydroxylase activity and its tissue and subcellular distributions were studied using rabbit, rat, bovine, chick, duck, guinea-pig and yellowtail. Vitamin D3 25-hydroxylase was found not only rabbit and bovine livers, kidneys and intestines, but also in lungs, pituitary glands, adrenocortices, ovaries, adipose tissues, blood vessels, brains and testes. Subcellular fractions of rabbit livers were examined for the presence of this activity and it was detected in the nuclear membranes, smooth and rough endoplasmic reticula, Golgi membranes and mitochondrial membranes. The activity in microsomes was induced by sodium phenobarbital, but not by beta-naphthoflavone and 3-methylcholanthrene, and was inhibited by isocyanide and metyrapone as inhibitor of cytochrome P-450.
利用兔、大鼠、牛、鸡、鸭、豚鼠和黄尾鱼研究了胆钙化醇（维生素D3）25-羟化酶活性及其组织和亚细胞分布。发现维生素D3 25-羟化酶不仅存在于兔和牛的肝脏、肾脏和肠道中，还存在于肺、垂体、肾上腺皮质、卵巢、脂肪组织、血管、脑和睾丸中。对兔肝脏的亚细胞组分进行了该活性检测，结果在核膜、滑面和粗面内质网、高尔基体膜和线粒体膜中检测到该活性。微粒体中的活性可被苯巴比妥钠诱导，但不能被β-萘黄酮和3-甲基胆蒽诱导，并且可被作为细胞色素P-450抑制剂的异腈和甲吡酮抑制。
