Visser B J, Labruyère W T, Spanjer W, Piek T
Comp Biochem Physiol B. 1983;75(3):523-30. doi: 10.1016/0305-0491(83)90370-x.
Two paralysing toxins (A-MTX and B-MTX) from extracts of Microbracon hebetor (Say) wasps were isolated and purified by gel chromatography, ion exchange chromatography and gel electrophoresis. Both toxins are labile proteins with molecular weights of 43,700 and 56,700, respectively, as estimated by gel chromatography, and with isoelectric pH of 6.85 and 6.62, respectively. Both toxins are inactivated by proteolytic enzymes and by dithiothreitol, but A-MTX appears to be more resistant than B-MTX. The relative amino acid compositions of both toxins show great similarity. The biological effects of the two toxins were identical to those previously found for crude toxin preparations.
从淡足侧沟茧蜂（Microbracon hebetor (Say)）提取物中分离出两种麻痹毒素（A - MTX和B - MTX），并通过凝胶色谱、离子交换色谱和凝胶电泳进行纯化。通过凝胶色谱估计，这两种毒素都是不稳定蛋白，分子量分别为43,700和56,700，等电点pH分别为6.85和6.62。两种毒素都可被蛋白水解酶和二硫苏糖醇灭活，但A - MTX似乎比B - MTX更具抗性。两种毒素的相对氨基酸组成显示出极大的相似性。这两种毒素的生物学效应与先前在粗毒素制剂中发现的效应相同。
