Gratzl M, Russell J T, Thorn N A
Experientia. 1983 Sep 15;39(9):1007-9. doi: 10.1007/BF01989775.
Slices of ox neurohypophyses and groups of isolated rat neurointermediate lobes were incubated in a medium containing horseradish peroxidase (HRP) and stimulated by high K+ concentrations in the medium. After washing, the tissue was homogenized and subjected to subcellular fractionation in a Percoll/sucrose gradient. HRP was exclusively taken up by particles banding at a low density of the gradient. The HRP containing particles located to this region included vacuoles of a size comparable to secretory vesicles.
将牛神经垂体切片和分离的大鼠神经中间叶组在含有辣根过氧化物酶（HRP）的培养基中孵育，并用培养基中的高钾浓度刺激。洗涤后，将组织匀浆并在Percoll/蔗糖梯度中进行亚细胞分级分离。HRP仅被梯度低密度处的颗粒摄取。位于该区域的含HRP颗粒包括大小与分泌囊泡相当的液泡。
