Godovac-Zimmermann J, Braunitzer G
Hoppe Seylers Z Physiol Chem. 1983 Jun;364(6):665-74. doi: 10.1515/bchm2.1983.364.1.665.
The complete amino-acid sequence of the major hemoglobin component (HbA) of the adult Northern Mallard (Anas platyrhynchos platyrhynchos) is presented. A minor component HbD was also detected but in low concentrations. The sequences of alpha A- and beta-chains were established by automatic Edman degradation on tryptic peptides and peptides obtained by specific chemical cleavages. The alignment of the peptides was performed by comparison with the alpha A- and beta-chains of Greylag Goose hemoglobin (Anser anser). Thereby an exchange of five positions in the alpha A-chains and three in the beta-chains was observed. No exchanges were found in the surroundings of the heme, in alpha 1 beta 2-contact points, or allosteric regulatory sites. In the alpha 1 beta 1-subunit interface one amino-acid residue in alpha A-chains and one in beta-chains are exchanged. By comparison with the amino-acid sequence derived from globin genes of Domestic Duck (Anas platyrhynchos), the alpha A-chains differ by two exchanges in the N-terminal region and the beta-chains by five exchanges the in C-terminal region. The comparison of the amino-acid sequence derived from alpha A-globin gene of the Moscovy Duck (Cairina moschata) and alpha A-chains of the Northern Mallard, shows only one replacement.
本文给出了成年绿头鸭（Anas platyrhynchos platyrhynchos）主要血红蛋白成分（HbA）的完整氨基酸序列。还检测到一种次要成分HbD，但浓度较低。αA链和β链的序列通过对胰蛋白酶肽段以及特定化学裂解得到的肽段进行自动埃德曼降解来确定。通过与灰雁血红蛋白（Anser anser）的αA链和β链进行比较来进行肽段比对。由此观察到αA链中有五个位置发生了交换，β链中有三个位置发生了交换。在血红素周围、α1β2接触点或变构调节位点未发现交换。在α1β1亚基界面，αA链和β链各有一个氨基酸残基发生了交换。与家鸭（Anas platyrhynchos）球蛋白基因推导的氨基酸序列相比，αA链在N端区域有两处交换，β链在C端区域有五处交换。疣鼻栖鸭（Cairina moschata）αA球蛋白基因推导的氨基酸序列与绿头鸭αA链的比较仅显示一处替换。
