Born W, Ben-Nun A, Bamberger U, Nakayama M, Speth V, Sun D, Thornton M
Immunobiology. 1983 Jul;165(1):63-77. doi: 10.1016/S0171-2985(83)80047-3.
Murine thymus cells were maintained in vitro with supernatant-factors derived from Concanavalin-A-stimulated spleen cells. After an initial phase of vigorous proliferation, large granular cells (GC), which were not observed in fresh thymus cell preparations, appeared in these cultures. GC, derived from C3Hf/Tif-, BALB/c-, and C57BL/10-thymus cultures, could be slowly expanded and have been maintained as increasingly homogeneous (oligoclonal) lines for up to six months. During this time, other types of thymus cells died or were diluted out. Thymic GC differ functionally and histochemically from macrophages and mast cells. They do not phagocytize zymosan particles, bind opsonized SRBC, express nonspecific esterases or contain detectable amounts of histamine. GC share many features with natural killer (NK) cells and large granular lymphocytes (LGL). One morphologically representative line (C3Hf/Tif) had the following surface phenotype: Thy-1+, Lyt-1-, Lyt-2-, H-2K+, I-A-, asialo Gm1+. GC bind peanut agglutinin (PNA) on their surface and contain azurophilic granules. These cytoplasmic granules are considerably larger than those in LGL. Cells of a GC line derived from mouse strain C3Hf/Tif (H-2k) lysed the NK-sensitive YAC-1 (H-2a) and EL-4 (H-2b), but not the NK-insensitive P815 cells.
将小鼠胸腺细胞与伴刀豆球蛋白A刺激的脾细胞产生的上清液因子一起进行体外培养。在最初的旺盛增殖阶段之后，新鲜胸腺细胞制剂中未观察到的大颗粒细胞（GC）出现在这些培养物中。源自C3Hf/Tif-、BALB/c-和C57BL/10-胸腺培养物的GC可以缓慢扩增，并作为日益均匀（寡克隆）的细胞系维持长达六个月。在此期间，其他类型的胸腺细胞死亡或被稀释掉。胸腺GC在功能和组织化学上与巨噬细胞和肥大细胞不同。它们不吞噬酵母聚糖颗粒，不结合调理的绵羊红细胞，不表达非特异性酯酶，也不含有可检测量的组胺。GC与自然杀伤（NK）细胞和大颗粒淋巴细胞（LGL）有许多共同特征。一个形态学上有代表性的细胞系（C3Hf/Tif）具有以下表面表型：Thy-1+、Lyt-1-、Lyt-2-、H-2K+、I-A-、脱唾液酸Gm1+。GC在其表面结合花生凝集素（PNA）并含有嗜天青颗粒。这些细胞质颗粒比LGL中的颗粒大得多。源自小鼠品系C3Hf/Tif（H-2k）的GC细胞系裂解NK敏感的YAC-1（H-2a）和EL-4（H-2b）细胞，但不裂解NK不敏感的P815细胞。
