Marder P, Schmidtke J R
Immunopharmacology. 1983 Aug;6(2):155-66. doi: 10.1016/0162-3109(83)90008-5.
The inhibition of concanavalin A-induced human peripheral blood lymphocyte blastogenesis by methylprednisolone (MP) was studied by using flow cytometry and tritiated thymidine (3H-TdR) incorporation. Flow cytometric determinations of volume, low angle forward light scatter, and nucleic acid showed MP to be a potent inhibitor of blastogenesis. The effects were concentration-dependent and correlated with 3H-TdR uptake. By using the single cell analytic capability of flow cytometry, the target stages of the cell cycle where MP affects lymphocyte activation were determined. Evidence is presented that steroids can block both early and late phases of this process.
通过流式细胞术和氚标记胸腺嘧啶核苷（3H-TdR）掺入法，研究了甲基泼尼松龙（MP）对刀豆球蛋白A诱导的人外周血淋巴细胞增殖的抑制作用。流式细胞术对细胞体积、低角度前向光散射和核酸的测定表明，MP是一种有效的增殖抑制因子。其作用呈浓度依赖性，且与3H-TdR摄取相关。利用流式细胞术的单细胞分析能力，确定了MP影响淋巴细胞激活的细胞周期目标阶段。有证据表明，类固醇可阻断这一过程的早期和晚期阶段。
