Lundstrom R C
J Assoc Off Anal Chem. 1983 Jul;66(4):974-80.
Isoelectric focusing (IEF) is currently the most reliable method available for the identification of fish species. The high resolution of this method usually allows discrimination between even closely related species. One genus, the Sebastes, does present a problem however. Using both low and high resolution, IEF is unable to differentiate several species. Disc electrophoresis, used in an AOAC official final action method, does not differentiate the rockfish reliably. Using IEF, identical protein patterns were obtained for Pacific Ocean perch (Sebastes alutus), Bocaccio rockfish (S. paucispinis), and yelloweye rockfish (S. ruberrimus). A second group, comprised of silvergray rockfish (S. brevispinis), yellowtail rockfish (S. flavidus), black rockfish (S. melanops), and canary rockfish (S. pinniger), also has identical protein patterns. Widow rockfish (S. entomelas) and chilipepper rockfish (S. goodei) each had a unique pattern, different from the above 2 groups and from each other. The actual taxonomic relationships of these rockfish species are not clear and further work with IEF may help in this regard. Users of IEF and disc electrophoresis for identification purposes should be aware of this problem when working with the Sebastes.
等电聚焦（IEF）是目前可用于鱼类物种鉴定的最可靠方法。该方法的高分辨率通常能够区分甚至是亲缘关系很近的物种。然而，对于某一属鱼类，即平鲉属，却存在一个问题。无论是使用低分辨率还是高分辨率，IEF都无法区分该属中的几个物种。在AOAC官方最终采用的方法中使用的圆盘电泳也无法可靠地区分岩鱼。使用IEF时，太平洋鲈（Sebastes alutus）、博氏平鲉（S. paucispinis）和黄眼平鲉（S. ruberrimus）获得了相同的蛋白质图谱。由银灰平鲉（S. brevispinis）、黄尾平鲉（S. flavidus）、黑平鲉（S. melanops）和加那利平鲉（S. pinniger）组成的第二组也具有相同的蛋白质图谱。寡妇平鲉（S. entomelas）和智利平鲉（S. goodei）各自具有独特的图谱，与上述两组不同且彼此也不同。这些岩鱼物种的实际分类关系尚不清楚，在这方面进一步开展IEF研究可能会有所帮助。使用IEF和圆盘电泳进行鉴定的人员在处理平鲉属鱼类时应意识到这一问题
