Mogami Y, Takahashi K
J Cell Sci. 1983 May;61:107-21. doi: 10.1242/jcs.61.1.107.
Microtubule sliding was induced in axonemes obtained from isolated cilia of Paramecium caudatum when they were exposed to a reactivating solution containing ATP after mild treatment with trypsin. Over a very wide range of concentrations (1 nM-4 mM), Ca2+ in the reactivating solution had no effect on the proportion of axonemes that disintegrated as the result of microtubule sliding. Also, the velocity of sliding, determined by cinematography, and the polarity of the direction of sliding-force generation, determined by electron microscopy with regards to the base-to-tip axis of the cilium, were not affected by Ca2+. The results indicate that the Ca sensitivity, which is responsible for the ciliary reversal response, was removed from the axoneme, possibly as the result of trypsin treatment. It is thus unlikely that Ca sensitivity is attributable to the basic sliding machinery that powers ciliary movement.
在用胰蛋白酶进行温和处理后，将尾草履虫分离纤毛获得的轴丝暴露于含有ATP的再激活溶液中时，会诱导微管滑动。在非常宽的浓度范围（1 nM - 4 mM）内，再激活溶液中的Ca2+对因微管滑动而解体的轴丝比例没有影响。此外，通过电影摄影确定的滑动速度以及通过电子显微镜关于纤毛基部到尖端轴确定的滑动力产生方向的极性，均不受Ca2+影响。结果表明，负责纤毛反向反应的Ca敏感性可能由于胰蛋白酶处理而从轴丝中去除。因此，Ca敏感性不太可能归因于驱动纤毛运动的基本滑动机制。
