Hashimoto Y, Sugawara M, Endoh H
J Immunol Methods. 1983 Aug 26;62(2):155-62. doi: 10.1016/0022-1759(83)90242-9.
SH-bearing subunits of IgM antibody were coupled through the Fc portion to sonicated liposomes consisting of N-(m-maleimidobenzoyl)dipalmitoylphosphatidylethanolamine, dipalmitoylphosphatidylcholine and cholesterol. Liposomes coated with subunits of monoclonal IgM antibody against a mouse mammary tumor-associated antigen selectively bound to the antigenic target cells. Assays of the liposomes for complement dependent cytotoxicity and spleen cell binding revealed inactivation of the liposome-coupled antibodies in both assays.
IgM抗体的含巯基亚基通过Fc部分与由N-（间马来酰亚胺苯甲酰基）二棕榈酰磷脂酰乙醇胺、二棕榈酰磷脂酰胆碱和胆固醇组成的超声处理脂质体偶联。用抗小鼠乳腺肿瘤相关抗原的单克隆IgM抗体亚基包被的脂质体选择性地结合到抗原靶细胞上。对脂质体进行补体依赖性细胞毒性和脾细胞结合分析发现，在这两种分析中脂质体偶联抗体均失活。
