Watson P F, Anderson W J
J Reprod Fertil. 1983 Sep;69(1):229-35. doi: 10.1530/jrf.0.0690229.
Butylated hydroxytoluene (BHT) significantly reduced the degree of acrosome damage which occurred to ram spermatozoa during cold shock. A higher percentage of spermatozoa were motile after cold shock in the presence of BHT than in its absence, but it had little effect on the quality of motility or the percentage of cells which stained with eosin. A concentration of 2-4 mM-BHT provided the maximum response. No advantage was gained by using the solvent, dimethyl sulphoxide, as a vehicle to introduce BHT to the cells. An osmotic stress test after cold shock also failed to demonstrate any advantage of BHT. It is concluded that BHT provides little protection to the functional capacity of ram spermatozoa undergoing cold stress and is unlikely to be of benefit for the preservation of ram semen.
丁基羟基甲苯（BHT）显著降低了冷休克期间公羊精子发生的顶体损伤程度。在有BHT存在的情况下，冷休克后有活力的精子百分比高于无BHT时，但它对运动质量或伊红染色细胞的百分比影响很小。2-4 mM的BHT浓度可产生最大反应。使用溶剂二甲基亚砜作为将BHT引入细胞的载体并无优势。冷休克后的渗透压应激试验也未能证明BHT有任何优势。得出的结论是，BHT对遭受冷应激的公羊精子功能能力几乎没有保护作用，不太可能有利于公羊精液的保存。
