Cro阻遏蛋白与DNA复合物的晶体学数据。

Crystallographic data for complexes of the Cro repressor with DNA.

作者信息

Anderson W F, Cygler M, Vandonselaar M, Ohlendorf D H, Matthews B W, Kim J, Takeda Y

出版信息

J Mol Biol. 1983 Aug 25;168(4):903-6. doi: 10.1016/s0022-2836(83)80082-5.

Abstract

Complexes of the bacteriophage lambda Cro repressor with two DNA duplexes have been crystallized. The DNA sequences are equimolar mixtures of ApTpCpApCpC and its complementary strand and ApCpCpGpCpApApGpG and its complementary strand, which are both parts of the lambda OR3 operator. The space group of both co-crystals is C2221 with cell dimensions a = 81.1 A, b = 89.2 A, and c = 80.0 A. Analysis of dissolved crystals shows that they respectively contain approximately two hexamers per Cro dimer and one nonamer per dimer. The co-crystals diffract to about 3 A resolution and appear suitable for structural studies.

摘要

噬菌体λ Cro阻遏物与两条DNA双链体的复合物已被结晶。DNA序列是ApTpCpApCpC及其互补链和ApCpCpGpCpApApGpG及其互补链的等摩尔混合物，它们都是λ OR3操纵子的一部分。两种共晶体的空间群均为C2221，晶胞尺寸为a = 81.1 Å，b = 89.2 Å，c = 80.0 Å。对溶解晶体的分析表明，它们分别每个Cro二聚体含有约两个六聚体和每个二聚体含有一个九聚体。共晶体的衍射分辨率约为3 Å，似乎适合进行结构研究。