Shain S A, Moss A L, Boesel R W, Lancaster C M
J Steroid Biochem. 1983 Jul;19(1C):985-93. doi: 10.1016/0022-4731(83)90044-4.
We have examined testosterone regulation of differentiated androgen responsive ventral prostate function in aging AXC rats as a possible means of identifying age-related changes in prostate gene function. Concerning cellular content of functional macromolecules, we identified three categories of age-related change in ventral prostate: (1) diminutions completely reversible by short-term chronic testosterone treatment, as exemplified by androgen receptor content; (2) diminutions partially reversible by testosterone treatment, as exemplified by prolactin receptor content and L-ornithine-decarboxylase (ODC) activity; and (3) diminutions not reversed by exogenous testosterone treatment, as exemplified by S-adenosyl-L-methionine decarboxylase (AMDC) activity. A fourth category of alteration revealed apparent age-related changes in polyamine homeostasis. The anabolic products of ODC and AMDC activities, putrescine, spermidine and spermine, showed an age-related diminished dependence between the activities of ODC and AMDC and ventral prostate polyamine content. The possibility that these changes reflect age-related alterations in ventral prostate gene activity is under investigation.
我们研究了睾酮对衰老的AXC大鼠中分化的雄激素反应性腹侧前列腺功能的调节作用，以此作为识别前列腺基因功能中与年龄相关变化的一种可能方法。关于功能性大分子的细胞含量，我们在腹侧前列腺中确定了三类与年龄相关的变化：（1）短期慢性睾酮治疗可完全逆转的减少，以雄激素受体含量为例；（2）睾酮治疗可部分逆转的减少，以催乳素受体含量和L-鸟氨酸脱羧酶（ODC）活性为例；（3）外源性睾酮治疗不能逆转的减少，以S-腺苷-L-甲硫氨酸脱羧酶（AMDC）活性为例。第四类改变揭示了多胺稳态中明显的与年龄相关的变化。ODC和AMDC活性的合成产物腐胺、亚精胺和精胺显示，ODC和AMDC活性与腹侧前列腺多胺含量之间存在与年龄相关的依赖性降低。这些变化是否反映腹侧前列腺基因活性的与年龄相关的改变正在研究中。
