Lax E R, Tamulevicius P, Müller A, Schriefers H
J Steroid Biochem. 1983 Aug;19(2):1083-8. doi: 10.1016/0022-4731(83)90400-4.
Nuclear estrogen receptor concentrations in rat liver were determined by exchange assay. The concentration of estrogen receptors in nuclei from vehicle-treated male and female rats show age-dependent, but not sex-dependent variations in the course of life. Levels are highest during the perinatal period (approximately 1500 binding sites/nucleus), whereafter they decrease towards the onset of puberty (approximately 300 binding sites/nucleus) before rising again to reach the postpuberal maximum (approximately 800 binding sites/nucleus). Pregnancy further raised receptor concentrations in the last week of gestation when they reach approximately 1200 binding sites/nucleus. Studies with ethynylestradiol-treated rats demonstrated that virtually no translocation can be detected before the onset of puberty; thereafter the number of translocated receptors increases dramatically reaching a maximum (9000 binding sites/nucleus) between day 80 and 87 of life. The extractability of the nuclear receptors with 0.4 M KCl varies during the course of life. Extractability is very high (approximately 90%) up to about day 12 of life, but then decreases markedly (to approximately 70% in vehicle-treated and to approximately 50% in ethynylestradiol-treated rats) before the onset of puberty.
通过交换测定法测定大鼠肝脏中的核雌激素受体浓度。在整个生命过程中，给予溶剂处理的雄性和雌性大鼠细胞核中的雌激素受体浓度呈现出年龄依赖性变化，但无性别依赖性变化。在围产期水平最高（约1500个结合位点/细胞核），此后在青春期开始前下降（约300个结合位点/细胞核），然后再次上升至青春期后达到最大值（约800个结合位点/细胞核）。妊娠在妊娠最后一周进一步提高了受体浓度，此时达到约1200个结合位点/细胞核。对乙炔雌二醇处理的大鼠的研究表明，在青春期开始前几乎检测不到核转位；此后，核转位受体的数量急剧增加，在生命的第80至87天之间达到最大值（9000个结合位点/细胞核）。核受体用0.4M KCl的可提取性在生命过程中有所变化。在生命约第12天之前可提取性非常高（约90%），但在青春期开始前显著下降（在给予溶剂处理的大鼠中降至约70%，在乙炔雌二醇处理的大鼠中降至约50%）。
