Rousseau A, Dubois G, Gatt S
Neurochem Res. 1983 Apr;8(4):417-22. doi: 10.1007/BF00965098.
Rat Brain has a lipase which hydrolyzes diacylglycerol at an optimal pH of 4.8 (1). The subcellular distribution of this acid diacylglycerol lipase was studied in brain tissue of rats and mice; in the latter case neurological mutants and their normal controls were used. Several other acidic hydrolases were employed as normal controls were used. Several other acidic hydrolases were employed as lysosomal markers. In mouse brain, the specific activity which is about 50-100 times lower than in rat brain, was greatest in the lysosomal fraction. In contrast, no enrichment of DG-lipase was observed in any subcellular fraction of the active enzyme of rat brain. Activities were about equally distributed in the microsomal, myelin-synaptosomal and lysosomal fractions.
大鼠脑中有一种脂肪酶，其在最适pH值为4.8时水解二酰基甘油(1)。对大鼠和小鼠脑组织中这种酸性二酰基甘油脂肪酶的亚细胞分布进行了研究；在小鼠实验中使用了神经突变体及其正常对照。还使用了其他几种酸性水解酶作为正常对照。另外几种酸性水解酶被用作溶酶体标记物。在小鼠脑中，比大鼠脑低约50 - 100倍的比活性在溶酶体部分最高。相比之下，在大鼠脑的任何亚细胞部分均未观察到二酰基甘油脂肪酶活性酶的富集。其活性在微粒体、髓磷脂 - 突触体和溶酶体部分中分布大致相等。
