Douglas R
Pathology. 1983 Apr;15(2):183-7. doi: 10.3109/00313028309084710.
An investigation is reported into the error rate of the indirect antiglobulin test when random, weakly reactive samples are tested. Tests were completed by the majority of laboratory staff in 2 transfusion services and 3 major general hospital blood banks, during their routine daily work. A significant level of technical difficulty was revealed, from 5.2 to 60.8% of positive tests being called negative, and from 5.7 to 32.3% of negative tests being called positive. Reproducibility of weakly reacting duplicates was generally poor, as was the allocation of score values to the agglutination reactions. The significance of these findings relative to the content of quality control surveys is discussed.
本文报告了一项关于对随机抽取的弱反应性样本进行间接抗球蛋白试验时错误率的调查。试验由2家输血服务机构和3家大型综合医院血库的大多数实验室工作人员在日常工作中完成。结果显示存在显著的技术困难，5.2%至60.8%的阳性试验被判定为阴性，5.7%至32.3%的阴性试验被判定为阳性。弱反应性重复样本的可重复性普遍较差，凝集反应的评分分配也是如此。文中讨论了这些发现相对于质量控制调查内容的意义。
