Ball A, Barratt G M, Wills E D
Eur J Cancer Clin Oncol. 1982 May;18(5):489-94. doi: 10.1016/0277-5379(82)90118-3.
The lysosomal enzyme activity and membrane permeability of mouse C3H mammary tumours has been studied using quantitative cytochemical methods following irradiation of the tumours with doses of 1500, 3500 or 6000 rad gamma rays. No change in the lysosomal enzyme activity was observed immediately after irradiation, but increased enzyme activity and increased membrane permeability were observed 24 hr after irradiation with doses of 3500 or 6000 rad. Twenty-four hours after injection of prednisolone there was a marked increase of lysosomal membrane permeability and enzyme activity, and injection of prednisolone soon after irradiation enhanced the effect of irradiation. After a dose of 6000 rad and prednisolone, the lysosomal membrane permeability increased to 191% of the control and the enzyme activity to 326% of the value of the controlled tumours. Measurement of tumour size after irradiation or after a combined treatment with irradiation and prednisolone showed that a close correlation exists between tumours regression and lysosomal enzyme activity. The experiments support the view that lysosomal enzymes play an important role in tumour regression following irradiation.
采用定量细胞化学方法，研究了剂量为1500、3500或6000拉德γ射线照射小鼠C3H乳腺肿瘤后，其溶酶体酶活性和膜通透性的变化。照射后即刻未观察到溶酶体酶活性的变化，但在3500或6000拉德剂量照射24小时后，观察到酶活性增加和膜通透性增加。注射泼尼松龙24小时后，溶酶体膜通透性和酶活性显著增加，照射后不久注射泼尼松龙可增强照射效果。给予6000拉德剂量和泼尼松龙后，溶酶体膜通透性增加至对照的191%，酶活性增加至对照肿瘤值的326%。照射后或照射与泼尼松龙联合治疗后测量肿瘤大小，结果表明肿瘤消退与溶酶体酶活性之间存在密切相关性。这些实验支持了溶酶体酶在照射后肿瘤消退中起重要作用的观点。
