Rister M
Fortschr Med. 1982 Jul 8;100(26):1247-50.
The exposure of guinea pigs to 85% oxygen increased in alveolar macrophages and granulocytes the Superoxid Dismutase activity. This enzyme protects aerobic organisms against the toxic superoxide anion. In contrast both cell types exhibited a decrease in hydrogen peroxide metabolizing enzymes Catalase and Glutathione perioxidase. In addition, the animal exposure to FiO2 of 85% impaired various phagocytic functions. A decrease of adherence, chemotaxis, ingestion rates as well as the bacterialcidal activity was observed in alveolar macrophages and granulocytes. In alveolar macrophages the degranulation was also diminished. These phagocytic function defects are caused by a cytoskeleton alteration, consistent of the microtubulus and microfilaments. The guinea pig exposure to a FiO2 of 85% did enhance the number of alveolar macrophages and granulocytes demonstrating a patchy FITC-Concannavalin A fluorescence, which is associated with a disruption of the microtubulus and the microfilaments. The phagocytic defects described in alveolar macrophages and granulocytes obtained from guinea pigs exposed to a FiO2 of 85% are based upon a microtubulus and microfilament disturbance in both cell types.
将豚鼠暴露于85%的氧气中，可增加肺泡巨噬细胞和粒细胞中的超氧化物歧化酶活性。这种酶可保护需氧生物免受有毒超氧阴离子的侵害。相比之下，这两种细胞类型的过氧化氢代谢酶（过氧化氢酶和谷胱甘肽过氧化物酶）活性均降低。此外，将动物暴露于85%的吸入氧分数会损害各种吞噬功能。在肺泡巨噬细胞和粒细胞中观察到黏附、趋化性、摄取率以及杀菌活性均降低。在肺泡巨噬细胞中，脱颗粒也减少。这些吞噬功能缺陷是由细胞骨架改变引起的，细胞骨架由微管和微丝组成。将豚鼠暴露于85%的吸入氧分数确实会增加肺泡巨噬细胞和粒细胞的数量，这些细胞呈现出斑片状的异硫氰酸荧光素 - 伴刀豆球蛋白A荧光，这与微管和微丝的破坏有关。从暴露于85%吸入氧分数的豚鼠中获得的肺泡巨噬细胞和粒细胞中所描述的吞噬缺陷是基于这两种细胞类型中的微管和微丝紊乱。
