Keil T A
Tissue Cell. 1982;14(3):451-62. doi: 10.1016/0040-8166(82)90039-8.
Antennal olfactory hairs of Antheraea polyphemus were investigated by means of transmission electron microscopy. Adequate preservation of dendrites and extracellular pore tubules is obtained by mechanical opening of the hair lumen and subsequent chemical fixation. The dendritic membrane has a cell coat. The dendrites contain microfilamentous structures in addition to their cytoplasmatic microtubules. The extracellular pore tubules traverse the hair cuticle and reach into the hair lumen for maximally 350 nm. Their diameter varies between 20 and 40 nm, depending on the preparation method. They consist of an electron-dense wall and an electron-lucent core. The wall has a helical substructure and is covered with a fuzzy coat. Contacts of pore tubules and dendritic membranes occur wherever dendrites are near the inner surface of the hair cuticle. Some of the pore tubules terminate approximately at right angles on the dendritic membrane, others lie against the membrane. The contact seems to be made via the surface coats of the tubules and the membrane. The structure of pore tubules which had been negatively stained with uranyl acetate is similar to the conventionally thin-sectioned material. The observations provide support for earlier assumptions that pore tubules are the pathways by which odor molecules reach the dendritic membrane.
利用透射电子显微镜对多音天蚕的触角嗅觉毛进行了研究。通过机械打开毛腔并随后进行化学固定，可实现对树突和细胞外孔小管的充分保存。树突膜有一层细胞被。除了细胞质微管外，树突还含有微丝状结构。细胞外孔小管穿过毛表皮，最大可深入毛腔350纳米。其直径在20到40纳米之间变化，这取决于制备方法。它们由电子致密的壁和电子透明的核心组成。壁具有螺旋亚结构，并覆盖有一层模糊的被膜。只要树突靠近毛表皮的内表面，孔小管就会与树突膜接触。一些孔小管大约以直角终止于树突膜上，另一些则靠在膜上。这种接触似乎是通过小管和膜的表面被膜实现的。用醋酸铀酰负染的孔小管结构与传统超薄切片材料相似。这些观察结果为早期的假设提供了支持，即孔小管是气味分子到达树突膜的途径。
