Hörer O L, Zaharia C N
Virologie. 1982 Jul-Sep;33(3):211-7.
The solvent perturbation technique was applied to the study of a membrane model consisting of lysozyme and sodium dodecyl sulfate (SDS) micelles, in comparison with erythrocyte ghosts, in aqueous solutions supplemented with dimethyl-sulfoxide. By correlated fluorescence and light-scattering spectrophotometric determinations it was possible to demonstrate a nonmonotonous variation suggesting a phenomenon equivalent to a conformation transition. Such a transition was made evident by the direct action of the cis-[PtCl2(HN3)2]complex on the lysozyme--SDS system at concentrations higher than the critical micelle concentration and at a temperature of 40 degrees C.
在添加了二甲基亚砜的水溶液中,将溶剂扰动技术应用于由溶菌酶和十二烷基硫酸钠(SDS)胶束组成的膜模型的研究,并与红细胞血影进行比较。通过相关的荧光和光散射分光光度法测定,有可能证明一种非单调变化,这表明存在一种相当于构象转变的现象。当顺式-[PtCl2(HN3)2]配合物在高于临界胶束浓度且温度为40摄氏度的条件下直接作用于溶菌酶-SDS体系时,这种转变变得明显。