Diekman M A, Anderson L L
Biol Reprod. 1982 Nov;27(4):816-26. doi: 10.1095/biolreprod27.4.816.
Nuclear and cytoplasmic exchange assays were developed and validated to quantify receptors for estradiol-17 beta (E217 beta) and progesterone (P4) in hypothalamic and pituitary tissues of gilts before, during, and after treatment with pregnant mare's serum (PMS) and human chorionic gonadotropin (hCG). Prepubertal gilts, 5 months old, were assigned randomly to four treatments. One group of gilts received 500 IU PMS (Day 0) and were sacrificed 2 days later (2 days post-PMS); another group received 500 IU PMS on Days 0 and 2, and were sacrificed 4 days from the initial injection (4 days post-PMS). A third group of gilts received PMS (500 IU) on Days 0 and 2, 1000 IU hCG on Day 4, and were sacrificed 5 days after hCG (5 days post-hCG). Controls were given saline on Days 0, 2 and 4 and sacrificed on Day 6. In pituitary tissues, there were no significant changes in numbers of cytoplasmic E2 17 beta receptors, cytoplasmic P4 receptors, nuclear P4 receptors or nuclear E2 17 beta receptors among the control, 2 days post-PMS, 4 days post-PMS or 5 days post-hCG treatment groups. In hypothalamic tissues, no differences in cytoplasmic E2 17 beta receptors, cytoplasmic P4 receptors or nuclear P4 receptors were found among any of the treatments. Nuclear receptors for E2 17 beta in hypothalamic tissues were greater, however, in gilts 2 days post-PMS (P less than 0.05) than in controls or 5 days post-hCG gilts, but they were not different from gilts 4 days post-PMS. Follicular development and serum concentrations of E2 17 beta followed the expected patterns after PMS; only ovaries from hCG-treated pigs contained corpora lutea. Because the PMS-hCG regimen simulated the onset of puberty, it seems that gilts attain puberty without a significant change in the number of receptors for E2 17 beta and P4 in the pituitary or hypothalamus.
开发并验证了核质交换分析方法，以定量测定在接受孕马血清（PMS）和人绒毛膜促性腺激素（hCG）处理之前、期间和之后的后备母猪下丘脑和垂体组织中雌二醇-17β（E217β）和孕酮（P4）的受体。将5月龄的青春期前后备母猪随机分为四组进行处理。一组后备母猪接受500IU PMS（第0天），并在2天后（PMS后2天）处死；另一组在第0天和第2天接受500IU PMS，并在初次注射后4天（PMS后4天）处死。第三组后备母猪在第0天和第2天接受PMS（500IU），在第4天接受1000IU hCG，并在hCG后5天（hCG后5天）处死。对照组在第0、2和4天给予生理盐水，并在第6天处死。在垂体组织中，对照组、PMS后2天、PMS后4天或hCG后5天处理组之间，细胞质E2 17β受体、细胞质P4受体、核P4受体或核E2 17β受体的数量没有显著变化。在下丘脑组织中，任何处理组之间的细胞质E2 17β受体、细胞质P4受体或核P4受体均未发现差异。然而，下丘脑组织中E2 17β的核受体在PMS后2天的后备母猪中比对照组或hCG后5天的后备母猪更多（P<0.05），但与PMS后4天的后备母猪没有差异。PMS后卵泡发育和E2 17β的血清浓度遵循预期模式；只有接受hCG处理的猪的卵巢中有黄体。由于PMS-hCG方案模拟了青春期的开始，似乎后备母猪在青春期时垂体或下丘脑中E2 17β和P4受体的数量没有显著变化。
