Vonk R J, Yousef I M, Corriveau J P, Tuchweber B
Liver. 1982 Jun;2(2):133-40. doi: 10.1111/j.1600-0676.1982.tb00189.x.
The hepatotoxic effect of phalloidin, a mushroom poison, was examined in whole liver and in isolated liver cell plasma membranes. By electron microscopy, a thickening of the pericanalicular microfilamentous network was noted. Bile canalicular enriched plasma membrane fractions of livers from phalloidin-treated animals contained increased amounts of microfilaments. Analysis of the peptides in these fractions, by sodium dodecyl sulfate polyacrylamide gel electrophoresis, indicated that although the actin peptide bands were prominent, a band apparently corresponding to myosin was drastically reduced. The morphological change was accompanied by a reduced bile flow and biliary excretion of bile acids, proteins and the exogenous organic anion dibromosulphthalein. These changes may result from a modification of the microfilaments, suggesting a role of the microfilaments in the liver membrane structure and function.
对一种蘑菇毒素——鬼笔环肽的肝毒性作用，在全肝和分离的肝细胞质膜中进行了研究。通过电子显微镜观察，发现肝小管周围微丝网络增厚。经鬼笔环肽处理的动物肝脏中，富含胆小管的质膜部分含有数量增加的微丝。通过十二烷基硫酸钠聚丙烯酰胺凝胶电泳对这些部分中的肽进行分析表明，虽然肌动蛋白肽带很明显，但一条明显对应于肌球蛋白的带却大幅减少。形态学变化伴随着胆汁流量以及胆汁酸、蛋白质和外源性有机阴离子二溴磺酞的胆汁排泄减少。这些变化可能是由于微丝的改变所致，提示微丝在肝细胞膜结构和功能中发挥作用。
