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[来自鸡腺胃黏液中间区的细胞视黄醇结合蛋白]

[Cellular retinol-binding protein from the mucous intermediate zone of the glandular stomach of chickens].

作者信息

Chernukhina L A, Khalmuradov A G, Dusheĭko A A, Blazhevich M A

出版信息

Biokhimiia. 1982 Dec;47(12):2028-34.

PMID:6891603
Abstract

A protein which specifically binds [3H]retinol in vitro and has a sedimentation coefficient of 2S was isolated from the soluble fraction of chicken glandular stomach intermediate zone mucosa cells and purified to homogeneity. The purification procedure included gel filtration through Sephadex G-75 and G-50 and ion-exchange chromatography on DEAE-cellulose. The properties of the protein were compared to those of the retinol-binding protein from rat liver.

摘要

从鸡腺胃中间区黏膜细胞的可溶性部分中分离出一种在体外能特异性结合[3H]视黄醇且沉降系数为2S的蛋白质,并将其纯化至同质。纯化过程包括通过Sephadex G - 75和G - 50进行凝胶过滤以及在DEAE - 纤维素上进行离子交换色谱。将该蛋白质的特性与大鼠肝脏视黄醇结合蛋白的特性进行了比较。

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