Sala M, Gu Z G, Moens G, Chouroulinkov I
Eur J Cancer Clin Oncol. 1982 Dec;18(12):1337-44. doi: 10.1016/0277-5379(82)90138-9.
Tris(2,3-dibromopropyl)phosphate (Tris-BP) and Tris(2-chloroethyl) orthophosphate (Genomoll P) were analyzed for mutagenic and carcinogenic activity in several in vitro and in vivo mammalian systems. In the in vitro tests both Tris-BP and Genomoll P increased sister chromatid exchanges in V79 cells with a dose-response relationship for Tris-BP. The mutation assays using the same cells (HGPRT locus) were negative. Both compounds showed positive results in the transformation of Syrian hamster embryo cells. A very low frequency of transformation in the C3H10T1/2 cells was obtained; we consider this result essentially negative. In the in vivo assays Tris-BP gave positive and Genomoll P questionable results in the micronucleus test performed on Chinese hamsters. Both gave negative results in short-term skin tests. In the long-term skin tests, Tris-BP showed an initiating activity which was not observed with Genomoll P. When they were used as promoters both chemicals increased the incidence of lung adenomas in mice. Comparatively, Genomoll P is far less hazardous than Tris-BP.
对磷酸三（2,3 - 二溴丙基）酯（Tris - BP）和磷酸三（2 - 氯乙基）酯（Genomoll P）在多种体外和体内哺乳动物系统中进行了致突变和致癌活性分析。在体外试验中，Tris - BP和Genomoll P均增加了V79细胞中的姐妹染色单体交换，且Tris - BP存在剂量反应关系。使用相同细胞（HGPRT位点）进行的突变试验结果为阴性。两种化合物在叙利亚仓鼠胚胎细胞转化试验中均呈阳性结果。在C3H10T1/2细胞中获得的转化频率非常低；我们认为该结果基本为阴性。在体内试验中，Tris - BP在中国仓鼠进行的微核试验中呈阳性结果，而Genomoll P的结果存疑。两者在短期皮肤试验中均呈阴性结果。在长期皮肤试验中，Tris - BP表现出启动活性，而Genomoll P未观察到该活性。当用作促癌剂时，两种化学物质均增加了小鼠肺腺瘤的发生率。相比之下，Genomoll P的危害远低于Tris - BP。
