Marchalonis J J, Warr G W
Dev Comp Immunol. 1978 Jul;2(3):443-59. doi: 10.1016/s0145-305x(78)80006-8.
A method is described which allows the isolation of small quantities of DNP-binding antibodies of both high- and low-molecular-weight classes. The only detectable DNP-binding protein in the serum of placoderm-derived vertebrates was immunoglobulin in nature, and of the order of less than or equal to 1% total serum immunoglobulin. Proteins in the serum of the urochordate Pyura stolonifera, which bind to both DNP and erythrocyte antigens, showed charge heterogeneity and an apparent subunit molecular weight of approximately 65,000 to 70,000 daltons.
本文描述了一种方法，该方法可分离出少量高分子量和低分子量类别的二硝基苯酚（DNP）结合抗体。在源自盾皮鱼纲的脊椎动物血清中，唯一可检测到的DNP结合蛋白本质上是免疫球蛋白，占血清总免疫球蛋白的比例小于或等于1%。尾索动物柄海鞘血清中与DNP和红细胞抗原均结合的蛋白质表现出电荷异质性，其表观亚基分子量约为65,000至70,000道尔顿。
