缺氧和寒冷对大鼠肺表面活性物质磷脂成分的联合作用。

Combined effect of hypoxia and cold on the phospholipid composition of lung surfactant in rats.

作者信息

Kumar R, Hegde K S, Krishna B, Sharma R S

出版信息

Aviat Space Environ Med. 1980 May;51(5):459-62.

Abstract

The phsopholipid composition of lung tissue and lung lavage in rats exposed to acute hypoxia, chronic hypoxia, and acute and chronic hypoxia associated with cold has been estimated and compared with controls. Different fractions of phospholipids were separated by thin layer chromatography. Results showed that acute hypoxia lowered phospholipids in lung lavage but superimposition of cold lowered phospholipids both in lung tissue and lavage. In chronic hypoxia, phospholipid contents of lung tissue and lavage decreased while the addition of cold showed no further reduction in lung tissue phospholipids; on the contrary phosphatidyl choline fraction of lung lavage increased. It is concluded that the effect of hypoxia alone in lowering surfactant is related to the duration of exposure. Cold with hypoxia lowers phospholipids in the acute stage; in the chronic stage; it does not further reduce phospholipids.

摘要

已对暴露于急性缺氧、慢性缺氧以及与寒冷相关的急性和慢性缺氧环境下的大鼠肺组织和肺灌洗液中的磷脂成分进行了估算，并与对照组进行了比较。通过薄层色谱法分离出不同的磷脂组分。结果显示，急性缺氧会降低肺灌洗液中的磷脂含量，但寒冷叠加会使肺组织和肺灌洗液中的磷脂含量均降低。在慢性缺氧状态下，肺组织和肺灌洗液中的磷脂含量下降，而寒冷的叠加并未使肺组织中的磷脂含量进一步降低；相反，肺灌洗液中的磷脂酰胆碱组分增加。得出的结论是，单纯缺氧降低表面活性剂的作用与暴露持续时间有关。缺氧与寒冷在急性期会降低磷脂含量；在慢性期，寒冷不会进一步降低磷脂含量。