大鼠肠上皮中肌球蛋白、肌动蛋白和原肌球蛋白的定位：光镜和电镜水平的免疫组织化学研究
Localization of myosin, actin, and tropomyosin in rat intestinal epithelium: immunohistochemical studies at the light and electron microscope levels.
作者信息
Drenckhahn D, Gröschel-Stewart U
出版信息
J Cell Biol. 1980 Aug;86(2):475-82. doi: 10.1083/jcb.86.2.475.
Abstract
Myosin, tropomyosin, and actin were localized in the epithelial cells of rat intestine by means of specific antibodies to chicken gizzard smooth muscle myosin, tropomyosin, and actin by immunohistochemical studies at both the light and electron microscope levels (unlabeled antibody enzyme technique). The pattern of antibody staining was the following (a) Anti-actin was associated with the microfilament bundles of the microvilli in their entire length, as well as with the microfilament network in the terminal web. (b) Anti-myosin was concentrated along the rootlets of the microvillar microfilament bundles and within the filamentous feltwork forming the terminal web. (c) Anti-tropomyosin showed a distribution similar to that of anti-myosin. In addition, the three antibodies also labeled the subplasmalemmal web underneath the cell membrane bordering on the basal lamina. Utilizing the above ultrastructural findings, we wish to propose a functional model of microvillar contraction.
摘要
通过使用针对鸡砂囊平滑肌肌球蛋白、原肌球蛋白和肌动蛋白的特异性抗体，采用光镜和电镜水平的免疫组织化学研究（未标记抗体酶技术），在大鼠肠道上皮细胞中定位了肌球蛋白、原肌球蛋白和肌动蛋白。抗体染色模式如下：（a）抗肌动蛋白与微绒毛的微丝束全长以及终末网中的微丝网络相关。（b）抗肌球蛋白集中在微绒毛微丝束的根部以及形成终末网的丝状网络内。（c）抗原肌球蛋白显示出与抗肌球蛋白相似的分布。此外，这三种抗体还标记了与基膜相邻的细胞膜下方的亚质膜网。利用上述超微结构发现，我们希望提出一个微绒毛收缩的功能模型。
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Renewal of chief cells and goblet cells in the small intestine as shown by radioautography after injection of thymidine-H3 into mice.给小鼠注射氚标记胸腺嘧啶核苷后，通过放射自显影术显示小肠主细胞和杯状细胞的更新情况。
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