Cameron D G, Casal H L, Mantsch H H
Biochemistry. 1980 Aug 5;19(16):3665-72. doi: 10.1021/bi00557a005.
Fourier transform infrared spectroscopy has been used to study the infrared-active acyl chain vibrational modes of fully hydrated multibilayers of 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (L-DPPC) over the temperature range 0-55 degrees C. Frequencies, bandwidths, and other spectral parameters were measured as a function of temperature for the methylene scissoring, rocking, and wagging modes, as well as for the C-H stretching modes, and they were used to monitor the packing of the acyl chains. Particular emphasis was placed on determining the nature of the pretransition event. It is shown that between 36 and 38 degrees C the spectral changes are indicative of a phase change in the acyl chain packing from an orthorhombid to a hexagonal subcell. It is also concluded that in the gel phase, at all temperatures below the main transition, the acyl chains are predominantly in all-trans conformations and that the temperature-dependent variations of spectral parameters result from changes in interchain interactions.
傅里叶变换红外光谱已被用于研究1,2 - 二棕榈酰 - sn - 甘油 - 3 - 磷酸胆碱（L - DPPC）完全水合多层膜在0至55摄氏度温度范围内的红外活性酰基链振动模式。测量了亚甲基剪式、摇摆和摆动模式以及C - H伸缩模式的频率、带宽和其他光谱参数随温度的变化，并用于监测酰基链的堆积情况。特别强调了确定预转变事件的性质。结果表明，在36至38摄氏度之间，光谱变化表明酰基链堆积从正交晶胞到六方亚晶胞的相变。还得出结论，在凝胶相中，在低于主要转变的所有温度下，酰基链主要处于全反式构象，光谱参数随温度的变化是由链间相互作用的变化引起的。
