Kristofferson D, Karr T L, Purich D L
J Biol Chem. 1980 Sep 25;255(18):8567-72.
This report presents a kinetic model for endwise depolymerization of linear, self-assembling protein systems. We develop a reliable method for predicting the shape of depolymerization curves (remaining polymer weight versus time) on the basis of the initial polymer length distribution. Computer simulations are used to illustrate changes in the polymer length distribution and average polymer weight during disassembly. In addition, our method provides an accurate determination of the microscopic rate constant for subunit release. Application of this analysis to dilution-induced and cold induced disassembly of microtubules is illustrated in the preceding paper (Karr, T.L., Kristofferson, D., and Purich, D.L. (1980) J. Biol. Chem. 255, 8560-8566). A survey of other possible applications of this treatment to microtubules, flagella, F-actin, and tobacco mosaic virus protein is included.
本报告提出了一种线性自组装蛋白质系统末端解聚的动力学模型。我们基于初始聚合物长度分布开发了一种可靠的方法来预测解聚曲线的形状（剩余聚合物重量与时间的关系）。计算机模拟用于说明拆卸过程中聚合物长度分布和平均聚合物重量的变化。此外，我们的方法能够准确测定亚基释放的微观速率常数。前文（Karr, T.L., Kristofferson, D., and Purich, D.L. (1980) J. Biol. Chem. 255, 8560 - 8566）展示了该分析在微管稀释诱导和解冷诱导解聚中的应用。还包括对该处理方法在微管、鞭毛、F - 肌动蛋白和烟草花叶病毒蛋白等其他可能应用的综述。
