一种能区分细胞质肌动蛋白和骨骼肌肌动蛋白的抗肌动蛋白抗体。
An antiactin antibody that distinguishes between cytoplasmic and skeletal muscle actins.
作者信息
Lubit B W, Schwartz J H
出版信息
J Cell Biol. 1980 Sep;86(3):891-7. doi: 10.1083/jcb.86.3.891.
Abstract
We elicited antibodies in rabbits to actin purified from body wall muscle of the marine mollusc, Aplysia californica. We found that this antiactin has an unusual specificity: in addition to reacting with the immunogen, it recognizes cytoplasmic vertebrate actins but not myofibrillar actin. Radioimmunoassay showed little or no cross-reaction with actin purified from either chicken gizzard or rabbit skeletal muscle. Immunocytochemical studies with human fibroblasts and L6 myoblasts revealed intense staining of typical cytoplasmic cables. Myofibrils were not stained after treatment of human and frog skeletal muscle with the antibody, although the distribution of immunofluorescence suggested that cytoplasmic actin is associated with membrane systems in the muscle fiber. The antibody may therefore be especially suited for studying the localization of cytoplasmic actin in skeletal muscle cells even in the presence of a great excess of the myofibrillar form.
摘要
我们在兔子体内诱导产生了针对从海洋软体动物加州海兔体壁肌肉中纯化出的肌动蛋白的抗体。我们发现这种抗肌动蛋白具有不同寻常的特异性：除了与免疫原发生反应外，它还能识别脊椎动物的细胞质肌动蛋白，但不能识别肌原纤维肌动蛋白。放射免疫分析表明，它与从鸡胗或兔骨骼肌中纯化出的肌动蛋白几乎没有交叉反应。对人成纤维细胞和L6成肌细胞进行的免疫细胞化学研究显示，典型的细胞质束呈现强烈染色。用该抗体处理人和青蛙的骨骼肌后，肌原纤维未被染色，尽管免疫荧光的分布表明细胞质肌动蛋白与肌纤维中的膜系统相关。因此，即使存在大量的肌原纤维形式，该抗体可能特别适合用于研究骨骼肌细胞中细胞质肌动蛋白的定位。
相似文献
1
An antiactin antibody that distinguishes between cytoplasmic and skeletal muscle actins.一种能区分细胞质肌动蛋白和骨骼肌肌动蛋白的抗肌动蛋白抗体。
J Cell Biol. 1980 Sep;86(3):891-7. doi: 10.1083/jcb.86.3.891.
2
Immunological characterization of an anti-actin antibody specific for cytoplasmic actins and its use for the immunocytological localization of actin in Aplysia nervous tissue.
J Histochem Cytochem. 1983 Jun;31(6):728-36. doi: 10.1177/31.6.6404982.
3
Subcellular sorting of isoactins: selective association of gamma actin with skeletal muscle mitochondria.肌动蛋白异构体的亚细胞分选：γ-肌动蛋白与骨骼肌线粒体的选择性结合。
Cell. 1983 Apr;32(4):1093-103. doi: 10.1016/0092-8674(83)90293-3.
4
Immunofluorescence comparisons of anti-actin specificity.抗肌动蛋白特异性的免疫荧光比较。
Eur J Cell Biol. 1982 Aug;28(1):115-21.
5
Peptide antibody specific for the amino terminus of skeletal muscle alpha-actin.对骨骼肌α-肌动蛋白氨基末端具有特异性的肽抗体。
Proc Natl Acad Sci U S A. 1983 Mar;80(6):1506-10. doi: 10.1073/pnas.80.6.1506.
6
Immunological differences between actins from cardiac muscle, skeletal muscle, and brain.心肌、骨骼肌和脑肌动蛋白之间的免疫学差异。
Proc Natl Acad Sci U S A. 1980 Apr;77(4):2069-73. doi: 10.1073/pnas.77.4.2069.
7
A simple procedure to produce monospecific polyclonal antibodies of high affinity against actin from muscular sources.一种制备针对肌肉来源肌动蛋白的高亲和力单特异性多克隆抗体的简单方法。
Eur J Cell Biol. 1989 Oct;50(1):220-9.
8
Localization of CapZ during myofibrillogenesis in cultured chicken muscle.培养的鸡肌肉肌原纤维生成过程中CapZ的定位
Cell Motil Cytoskeleton. 1993;25(4):317-35. doi: 10.1002/cm.970250403.
9
Microvascular pericytes contain muscle and nonmuscle actins.微血管周细胞含有肌肉型和非肌肉型肌动蛋白。
J Cell Biol. 1985 Jul;101(1):43-52. doi: 10.1083/jcb.101.1.43.
10
Two monoclonal antibodies to actin: one muscle selective and one generally reactive.两种针对肌动蛋白的单克隆抗体：一种具有肌肉选择性，另一种具有普遍反应性。
Cell Motil Cytoskeleton. 1988;10(3):349-62. doi: 10.1002/cm.970100302.
引用本文的文献
1
Multiple Mechanisms to Regulate Actin Functions: "Fundamental" Versus Lineage-Specific Mechanisms and Hierarchical Relationships.调控肌动蛋白功能的多种机制：“基础”机制与谱系特异性机制及层级关系
Biomolecules. 2025 Feb 13;15(2):279. doi: 10.3390/biom15020279.
2
Muscle cofilin alters neuromuscular junction postsynaptic development to strengthen functional neurotransmission.肌动蛋白丝结合蛋白改变神经肌肉接头的突触后发育，从而增强功能性神经递质传递。
Development. 2024 Jul 1;151(13). doi: 10.1242/dev.202558. Epub 2024 Jul 8.
3
Quadriceps myopathy caused by skeletal muscle-specific ablation of β(cyto)-actin.骨骼肌特异性消融β（细胞）肌动蛋白导致的四头肌肌病。
J Cell Sci. 2011 Mar 15;124(Pt 6):951-7. doi: 10.1242/jcs.079848. Epub 2011 Feb 15.
4
Diverse roles of the actin cytoskeleton in striated muscle.肌动蛋白细胞骨架在横纹肌中的多种作用。
J Muscle Res Cell Motil. 2009;30(5-6):187-97. doi: 10.1007/s10974-009-9193-x. Epub 2009 Dec 8.
5
Cytoplasmic gamma-actin contributes to a compensatory remodeling response in dystrophin-deficient muscle.细胞质γ-肌动蛋白有助于肌营养不良蛋白缺乏的肌肉产生代偿性重塑反应。
Proc Natl Acad Sci U S A. 2006 Apr 4;103(14):5385-90. doi: 10.1073/pnas.0600980103. Epub 2006 Mar 24.
6
Differential epitope tagging of actin in transformed Drosophila produces distinct effects on myofibril assembly and function of the indirect flight muscle.在转化的果蝇中对肌动蛋白进行差异表位标记，会对肌原纤维组装和间接飞行肌的功能产生不同影响。
Mol Biol Cell. 1999 Jan;10(1):135-49. doi: 10.1091/mbc.10.1.135.
7
Dominant negative effect of cytoplasmic actin isoproteins on cardiomyocyte cytoarchitecture and function.细胞质肌动蛋白同工蛋白对心肌细胞细胞结构和功能的显性负效应。
J Cell Biol. 1995 Dec;131(6 Pt 2):1759-73. doi: 10.1083/jcb.131.6.1759.
8
Indirect association of ezrin with F-actin: isoform specificity and calcium sensitivity.埃兹蛋白与丝状肌动蛋白的间接关联：同工型特异性和钙敏感性。
J Cell Biol. 1995 Mar;128(5):837-48. doi: 10.1083/jcb.128.5.837.
9
A low molecular weight tracer molecule for immunocytochemistry. Identification of cytoplasmic actin.
Experientia. 1981 Oct 15;37(10):1066-8. doi: 10.1007/BF02085011.
10
Cytoplasmic actin in postsynaptic structures at the neuromuscular junction.神经肌肉接头处突触后结构中的细胞质肌动蛋白。
J Cell Biol. 1981 Sep;90(3):789-92. doi: 10.1083/jcb.90.3.789.
本文引用的文献
1
Structural organization of the Z-line protein, alpha-actinin, in developing skeletal muscle cells.发育中的骨骼肌细胞中Z线蛋白α-辅肌动蛋白的结构组织
Dev Biol. 1980 Mar;75(1):231-8. doi: 10.1016/0012-1606(80)90158-x.
2
The reliability of molecular weight determinations by dodecyl sulfate-polyacrylamide gel electrophoresis.通过十二烷基硫酸钠-聚丙烯酰胺凝胶电泳测定分子量的可靠性。
J Biol Chem. 1969 Aug 25;244(16):4406-12.
3
Mathematical analysis of kinetics of radioligand assays: improved sensitivity obtained by delayed addition of labeled ligand.放射性配体分析动力学的数学分析：通过延迟添加标记配体提高灵敏度。
J Clin Endocrinol Metab. 1971 Aug;33(2):343-55. doi: 10.1210/jcem-33-2-343.
4
A monolayer preparation of innervated skeletal muscle fibres of the m. cutaneus pectoris of the frog.青蛙胸皮肌有神经支配的骨骼肌纤维的单层制备物。
Pflugers Arch. 1974 Apr 22;348(3):257-62. doi: 10.1007/BF00587416.
5
6
Paramyosin and the filaments of molluscan "catch" muscles. II. Native filaments: isolation and characterization.副肌球蛋白与软体动物“捕获”肌肉的细丝。II. 天然细丝：分离与特性
J Mol Biol. 1971 Mar 14;56(2):239-58. doi: 10.1016/0022-2836(71)90462-1.
7
Actin antibody: the specific visualization of actin filaments in non-muscle cells.肌动蛋白抗体：非肌肉细胞中肌动蛋白丝的特异性可视化。
Proc Natl Acad Sci U S A. 1974 Jun;71(6):2268-72. doi: 10.1073/pnas.71.6.2268.
8
The cross-linking of proteins with glutaraldehyde and its use for the preparation of immunoadsorbents.蛋白质与戊二醛的交联及其在免疫吸附剂制备中的应用。
Immunochemistry. 1969 Jan;6(1):53-66. doi: 10.1016/0019-2791(69)90178-5.
9
Actin is the naturally occurring inhibitor of deoxyribonuclease I.肌动蛋白是脱氧核糖核酸酶I的天然抑制剂。
Proc Natl Acad Sci U S A. 1974 Dec;71(12):4742-6. doi: 10.1073/pnas.71.12.4742.
Zotero 插件