Lizardi P M
J Cell Biol. 1980 Oct;87(1):292-6. doi: 10.1083/jcb.87.1.292.
Hydroxystilbamidine isethionate, a dye capable of binding to both DNA and RNA, has been found to be a powerful inhibitor of cellular ribonucleases. A procedure has been developed that, with the aid of this compound, permits the preparative isolation of giant silk fibroin polyribosomes from the posterior silk gland of Bombyx mori. The polyribosomes contain approximately 45-112 ribosomal particles, as judged by electron microscopy. Treatment of giant fibroin polyribosomes with EDTA releases a particle that sediments at 125S. This mRNP particle contains biologically active silk fibroin mRNA, as judged by cell-free translation in an mRNA-dependent reticulocyte cell-free system.
羟乙磺酸羟 stilbamidine，一种能够与 DNA 和 RNA 结合的染料，已被发现是细胞核糖核酸酶的强力抑制剂。已开发出一种方法，借助该化合物可从家蚕后部丝腺中制备分离出巨大的丝素蛋白多核糖体。通过电子显微镜判断，这些多核糖体含有约 45 - 112 个核糖体颗粒。用 EDTA 处理巨大的丝素蛋白多核糖体可释放出一个沉降系数为 125S 的颗粒。在依赖 mRNA 的网织红细胞无细胞系统中进行无细胞翻译判断，该 mRNP 颗粒含有具有生物活性的丝素蛋白 mRNA。
