Ryan S F, Liau D F, Bell A L, Hashim S A, Barrett C R
Am Rev Respir Dis. 1981 Feb;123(2):200-4. doi: 10.1164/arrd.1981.123.2.200.
Acute alveolar injury was induced in dogs by subcutaneous injection of 8 mg of N-nitroso-N-methylurethane per kg of body weight. Two to seven days after the injections, the change in respiratory compliance was measured, alveolar and lung tissue disaturated phosphatidyl choline (DSPC) were quantified, and fatty acid patterns of alveolar phosphatidyl choline were determined. Progressive decreases occurred in respiratory compliance and in alveolar and tissue DSPC. Mean alveolar DSPC decreases occurred in respiratory compliance and in alveolar and tissue DSPC. Mean alveolar DSPC decreased to 22% (p less than 0.001) of that of the control study and significant linear correlation (p = 0.02) between respiratory compliance and alveolar DSPC quantity was found. Mean tissue DSPC decreased to 60% of that of the control study (p less than 0.001) and reductions in alveolar and in type II cell DSPC were estimated to be nearly equivalent. The proportion of palmitate in lavage phosphatidyl choline was increased by a mean of 9%. These studies demonstrated a decrease in surfactant lipids correlated with decreased compliances in acute alveolar injury and suggested that qualitative and quantitative changes in surfactant result from injury to alveolar type II cells.
通过每千克体重皮下注射8毫克N-亚硝基-N-甲基氨基甲酸酯诱导犬急性肺泡损伤。注射后2至7天，测量呼吸顺应性的变化，定量肺泡和肺组织不饱和磷脂酰胆碱（DSPC），并测定肺泡磷脂酰胆碱的脂肪酸模式。呼吸顺应性以及肺泡和组织DSPC均出现进行性下降。平均肺泡DSPC下降至对照研究的22%（p<0.001），并且发现呼吸顺应性与肺泡DSPC量之间存在显著线性相关性（p = 0.02）。平均组织DSPC下降至对照研究的60%（p<0.001），并且估计肺泡和II型细胞DSPC的减少几乎相等。灌洗磷脂酰胆碱中棕榈酸酯的比例平均增加了9%。这些研究表明，急性肺泡损伤中表面活性物质脂质减少与顺应性降低相关，并提示表面活性物质的定性和定量变化是由肺泡II型细胞损伤引起的。
