Miura K, Nakamura H, Tamura Z
J Chromatogr. 1981 Jun 19;210(3):461-7. doi: 10.1016/s0021-9673(00)80338-6.
The parallel flow dialysis technique was improved for application to the detection of drug-binding proteins in a column chromatographic effluent. To prevent the baseline drift, the pressures of both protein and drug channels were maintained equal during chromatography, and Brij-35 was added to the solvents. The improved method was successfully applied to the detection of methyl orange-binding proteins in human serum and bromphenol blue-binding proteins in rat liver homogenate.
平行流透析技术得到改进，用于柱色谱流出物中药物结合蛋白的检测。为防止基线漂移，在色谱分析过程中，蛋白质通道和药物通道的压力保持相等，并向溶剂中添加了Brij-35。改进后的方法成功应用于检测人血清中的甲基橙结合蛋白和大鼠肝脏匀浆中的溴酚蓝结合蛋白。
