组织化学染色。其用于检测骨骼肌中的甘油醛-3-磷酸脱氢酶。
Histochemical staining. Its use for detection of glyceraldehyde-3-phosphate dehydrogenase in skeletal muscle.
作者信息
Brumback R A, Susag M E, Gerst J W
出版信息
Arch Pathol Lab Med. 1981 Aug;105(8):419-20.
Histochemical staining of glyceraldehyde-3-phosphate dehydrogenase activity in fresh-frozen muscle tissue may be performed using a beta-nicotinamide adenine dinucleotide (beta-NAD) reduced-dehydrogenase tetrazolium-reductase linked reaction. The phosphate-buffered incubating medium contains glyceraldehyde-3-phosphate, beta-NAD, nitro blue tetrazolium, and edetic acid. This technique produces a fine reticular pattern of blue staining in muscle fibers. In human muscle specimens, type I fibers stain darker than type II fibers.
可使用β-烟酰胺腺嘌呤二核苷酸（β-NAD）还原脱氢酶四氮唑还原酶连接反应对新鲜冷冻肌肉组织中的甘油醛-3-磷酸脱氢酶活性进行组织化学染色。磷酸盐缓冲孵育培养基含有甘油醛-3-磷酸、β-NAD、硝基蓝四氮唑和乙二胺四乙酸。该技术在肌肉纤维中产生蓝色染色的精细网状图案。在人体肌肉标本中，I型纤维比II型纤维染色更深。
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