Hughes P, Lowe C R, Sherwood R F
Biochim Biophys Acta. 1982 Jan 4;700(1):90-100. doi: 10.1016/0167-4838(82)90296-5.
Low concentrations of metal ions, particularly those of the first row transition series such as Zn2+, Co2+, Mn2+, Ni2+, Cu2+, and, to a lesser extent, the group IIA ions, Ca2+ and Mg2+, promotes binding of carboxypeptidase G2, alkaline phosphatase and yeast hexokinase to immobilized Procion Red H-8BN, Procion Yellow H-A and Cibacron Blue F3G-A respectively. The binding of ovalbumin to immobilized Cibacron Blue F3G-A and Procion Orange MX-G is selectively enhanced in the presence of AI3+. With ovalbumin and alkaline phosphatase, the effect is almost totally specific for both the metal ion and dye, whereas with carboxypeptidase G2 and hexokinase, metal ions such as Co2+, Ni2+, Mn2+, Cu2+, Ca2+ and Mg2+ also promote binding to varying degrees. Almost all other monovalent and trivalent metal ions appear to be ineffective. Metal ion-bound enzymes can subsequently be eluted with appropriate chelating agents of the amine, aminocarboxylate or substituted pyridine classes.
低浓度的金属离子，特别是第一排过渡系列的那些离子，如Zn2+、Co2+、Mn2+、Ni2+、Cu2+，以及在较小程度上的IIA族离子Ca2+和Mg2+，分别促进羧肽酶G2、碱性磷酸酶和酵母己糖激酶与固定化的普施安红H - 8BN、普施安黄H - A和汽巴克隆蓝F3G - A的结合。在Al3+存在的情况下，卵清蛋白与固定化的汽巴克隆蓝F3G - A和普施安橙MX - G的结合被选择性增强。对于卵清蛋白和碱性磷酸酶，这种效应几乎对金属离子和染料都具有完全特异性，而对于羧肽酶G2和己糖激酶，Co2+、Ni2+、Mn2+、Cu2+、Ca2+和Mg2+等金属离子也会不同程度地促进结合。几乎所有其他单价和三价金属离子似乎都没有效果。金属离子结合的酶随后可用胺类、氨基羧酸盐类或取代吡啶类的适当螯合剂洗脱。
