过敏反应迟缓反应物质与一些白三烯D4异构体的物理性质及酶促反应的比较研究

A comparative study of the physical properties and enzymatic reactions of slow reacting substance of anaphylaxis and some leukotriene D4 isomers.

作者信息

Baker S R, Boot J R, Osborne D J

出版信息

Prostaglandins. 1982 Apr;23(4):569-77. doi: 10.1016/0090-6980(82)90117-4.

Abstract

Eight synthetic isomers of 5-hydroxy-6-S-cysteinylglycine -7,9,11,14-eicosatetraenoic acid were compared with authentic guinea pig SRS-A using UV spectroscopy, high performance liquid chromatography and soybean lipoxygenase. It was found that only the 5S, 6R 7,9trans 11,14cis isomer was similar to SRS-A in all respects. The 5S,6R 7trans, 9,11,14 cis isomer shows similar UV and HPLC characteristics but differs in that it spontaneously undergoes a 1,7 hydride shift reaction and unexpectedly does not react with soybean lipoxygenase.

摘要

使用紫外光谱法、高效液相色谱法和大豆脂氧合酶，将8种5-羟基-6-S-半胱氨酰甘氨酸-7,9,11,14-二十碳四烯酸的合成异构体与豚鼠的天然慢反应物质（SRS-A）进行了比较。结果发现，只有5S, 6R 7,9反式 11,14顺式异构体在所有方面都与SRS-A相似。5S,6R 7反式, 9,11,14顺式异构体显示出相似的紫外和高效液相色谱特征，但不同之处在于它会自发进行1,7氢化物迁移反应，并且意外地不与大豆脂氧合酶发生反应。