Boulieu R, Bory C, Baltassat P, Gonnet C
J Chromatogr. 1982 Dec 10;233:131-40. doi: 10.1016/s0378-4347(00)81739-9.
A rapid and selective reversed-phase high-performance liquid chromatographic method for the simultaneous determination of hypoxanthine and xanthine in biological fluids was developed. The identification of hypoxanthine and xanthine was confirmed by xanthine oxidase reaction. This method was applied to the investigation of purine metabolism in subjects with xanthine oxidase deficiency or gout. Hypoxanthine concentrations three to ten times higher than those determined in plasma were found in erythrocyte samples from normal subjects and from patients with xanthine oxidase deficiency or hyperuricemia under allopurinol therapy.
建立了一种快速、选择性的反相高效液相色谱法，用于同时测定生物体液中的次黄嘌呤和黄嘌呤。通过黄嘌呤氧化酶反应确认了次黄嘌呤和黄嘌呤的鉴定。该方法应用于研究黄嘌呤氧化酶缺乏或痛风患者的嘌呤代谢。在正常受试者以及接受别嘌呤醇治疗的黄嘌呤氧化酶缺乏或高尿酸血症患者的红细胞样本中，发现次黄嘌呤浓度比血浆中测定的浓度高3至10倍。
