大鼠腹侧前列腺组织碎片对异柠檬酸的摄取及柠檬酸的生成

Isocitrate uptake and citrate production by rat ventral prostate fragments.

作者信息

Franklin R B, Costello L C

出版信息

Invest Urol. 1978 Jul;16(1):44-7.

Abstract

Prostatic aconitase was investigated utilizing fragments of rat ventral prostate. The rate of citrate formation from isocitrate was used as an index of aconitase activity. Inhibition of citrate formation from isocitrate by fluorocitrate indicated that aconitase activity was being measured. The results indicated that whereas both kidney and prostate formed citrate from isocitrate, the aconitase activity of prostate was independent of both medium isocitrate and citrate concentration. In addition the aconitase activity of prostate seemed to favor citrate formation to a much greater degree than kidney. The results showed that the aconitase catalyzed conversion of citrate to isocitrate is limited in prostate and suggested that the deficient citrate oxidation observed in prostate is the result of a unique aconitase activity.

摘要

利用大鼠腹侧前列腺组织片段对前列腺乌头酸酶进行了研究。以异柠檬酸生成柠檬酸的速率作为乌头酸酶活性指标。氟柠檬酸对异柠檬酸生成柠檬酸的抑制作用表明所测定的是乌头酸酶活性。结果表明，肾脏和前列腺均可由异柠檬酸生成柠檬酸，但前列腺的乌头酸酶活性与培养基中的异柠檬酸和柠檬酸浓度均无关。此外，前列腺的乌头酸酶活性似乎比肾脏更有利于柠檬酸的生成。结果显示，在前列腺中乌头酸酶催化的柠檬酸向异柠檬酸的转化受到限制，提示在前列腺中观察到的柠檬酸氧化不足是独特的乌头酸酶活性的结果。