Al-Mehdi M I
Histochemistry. 1978 Jul 12;56(3-4):317-27. doi: 10.1007/BF00495993.
Corpora lutea from rat ovaries at mid pregnancy were fixed by perfusion and studied by electron cytochemistry for localisation of four hydrolytic enzymes. Using the metal-salt methods for acid phosphatase and aryl sulphatases activity was localised in small and large lysosomes, multivesicular bodies, Golgi complex and within cisternae of endoplasmic reticulum. The azo-dye coupling method for Beta-glucuronidase was less satisfactory and gave positive results in lysosomes, lipids and in the globules within the mitochondrial matrix. The latter two localisation were probably associated with affinity of the naphthol AS-BI for lipid material. In addition to plasma membranes, the reaction product for alkaline phosphatase with the lead-salt method was seen in lysosomelike bodies, in smooth endoplasmic reticulum and in occasional Golgi elements of granulosa lutein and endothelial cells. Increased activity of lysosomal acid hydrolases occurs when regressive changes of lutein cells start at the end of gestation and this might probably reflect the initiation of lytic processes.
通过灌注法固定妊娠中期大鼠卵巢的黄体，并采用电子细胞化学方法研究四种水解酶的定位。利用金属盐法检测酸性磷酸酶和芳基硫酸酯酶，其活性定位于小和大的溶酶体、多囊泡体、高尔基体以及内质网池内。用于β - 葡萄糖醛酸酶的偶氮染料偶联法不太理想，在溶酶体、脂质以及线粒体基质内的小球体中呈阳性结果。后两种定位可能与萘酚AS - BI对脂质物质的亲和力有关。除质膜外，用铅盐法检测碱性磷酸酶的反应产物可见于黄体颗粒细胞和内皮细胞的类溶酶体、滑面内质网以及偶尔的高尔基体成分中。妊娠末期黄体细胞开始退行性变化时，溶酶体酸性水解酶活性增加，这可能反映了解溶过程的启动。
