Paavola L G
J Cell Biol. 1978 Oct;79(1):45-58. doi: 10.1083/jcb.79.1.45.
This study characterizes the cytochemical properties of the Golgi complex, the structure which corresponds to Golgi complex-endoplasmic reticulum-lysosomes (GERL), and the granule population in luteal cells of guinea pigs at the time of maximum progesterone secretion, in material fixed by vascular perfusion, a method particularly suited for preserving both fine structure and enzyme activity. The distribution of several marker enzymes was determined by electron microscope cytochemistry. Acid phosphatase (ACPase) and arylsulfatase were used to identify structures containing lysosomal proteins. To resolve specific problems, additional cytochemical markers were employed: localization of thiamine pyrophosphatase (TPPase) (in the Golgi complex) and alkaline phosphatase (ALPase) (a plasma membrane marker), and prolonged osmication (a generally accepted method of marking the outer cisterna of the Golgi complex). The results demonstrate that at the time of peak steroid secretion the Golgi complex in luteal cells, in marked contrast to that of most other cell types, typically displays intense ACPase activity in all of its cisternae. Similarly, all Golgi cisternae stain after prolonged osmication and may show TPPase activity. On the other hand, GERL in luteal cells of this age, unlike that in most cells, commonly shows low levels of, or lacks, ACPase activity. However, GERL resembles that of other cell types in being TPPase-negative and in being unstained by treatment with aqueous OsO4. GERL and some Golgi cisternae are reactive for ALPase. The granule population in luteal cells of this stage consists of lysosomes, multivesicular bodies, electrontransparent vacuoles, and microperoxisome-like bodies. These results form a base line with which luteolytic changes described in the companion study (Paavola, L.G. 1978. The corpus luteum of the guinea pig. III. Cytochemical studies on the Golgi complex and GERL during normal postpartum regression of luteal cells, emphasizing the origin of lysosomes and autophagic vacuoles. J. Cell. Biol. 79:59--73.) can be compared.
本研究通过血管灌注固定材料（一种特别适合保存精细结构和酶活性的方法），对豚鼠黄体细胞在孕酮分泌量最大时的高尔基体复合体（对应于高尔基体-内质网-溶酶体结构，即GERL）的细胞化学特性以及颗粒群体进行了表征。通过电子显微镜细胞化学确定了几种标记酶的分布。酸性磷酸酶（ACPase）和芳基硫酸酯酶用于识别含有溶酶体蛋白的结构。为解决特定问题，还采用了其他细胞化学标记：硫胺素焦磷酸酶（TPPase）（在高尔基体复合体中）和碱性磷酸酶（ALPase）（一种质膜标记）的定位，以及延长锇酸浸染时间（一种普遍认可的标记高尔基体复合体外侧潴泡的方法）。结果表明，在类固醇分泌高峰期，黄体细胞中的高尔基体复合体与大多数其他细胞类型的高尔基体复合体形成鲜明对比，其所有潴泡通常都显示出强烈的ACPase活性。同样，经过延长锇酸浸染后，所有高尔基体潴泡都会染色，并且可能显示TPPase活性。另一方面，这个年龄段的豚鼠黄体细胞中的GERL与大多数细胞中的GERL不同，通常显示出低水平的ACPase活性或缺乏ACPase活性。然而，GERL在对TPPase呈阴性以及经水性OsO4处理后不染色方面与其他细胞类型相似。GERL和一些高尔基体潴泡对ALPase有反应。这个阶段黄体细胞中的颗粒群体包括溶酶体、多泡体、电子透明液泡和微过氧化物酶体样小体。这些结果形成了一个基线，可用于与配套研究（Paavola, L.G. 1978.豚鼠黄体。III.黄体细胞正常产后退化过程中高尔基体复合体和GERL的细胞化学研究，重点关注溶酶体和自噬泡的起源。《细胞生物学杂志》79:59 - 73.）中描述的黄体溶解变化进行比较。
