Adachi K, Asakura T
J Biol Chem. 1978 Oct 10;253(19):6641-3.
Diluted solutions (less than 100 mg/dl) of deoxygenated sickle hemoglobin (Hb S) and Hb CHarlem were found to aggregate with a clear demonstration of a delay time when dissolved in concentrated phosphate buffer. The delay time could be shortened by increasing the concentration of either hemoglobin or phosphate. Upon cooling or bubbling with carbon monoxide, the aggregates melted quickly. The logarithmic plot of reciprocal delay time versus hemoglobin or phosphate concentration shows a linear line with a slope of 2.7 for hemoglobin and 44 for phosphate. This suggests that diluted solutions of Hb S aggregate by the nucleation mechanism which is similar to that reported in the gelation of concentrated Hb S solution.
发现脱氧镰状血红蛋白（Hb S）和Hb哈勒姆的稀释溶液（低于100 mg/dl）在溶解于浓缩磷酸盐缓冲液时会聚集，并且清楚地显示出延迟时间。通过增加血红蛋白或磷酸盐的浓度可以缩短延迟时间。冷却或用一氧化碳鼓泡后，聚集体会迅速融化。延迟时间的倒数与血红蛋白或磷酸盐浓度的对数图显示为一条直线，血红蛋白的斜率为2.7，磷酸盐的斜率为44。这表明Hb S的稀释溶液通过成核机制聚集，这与浓缩Hb S溶液凝胶化中报道的机制相似。
