Caple M B, Chow H, Strouse C E
J Biol Chem. 1978 Oct 10;253(19):6730-7.
Bacteriochlorophyll c, the light-harvesting pigment from the green sulfur bacteria Chlorobium limicola, has been resolved into over a dozen chemically similar components by a new high pressure reversed-phase chromatographic procedure based on a stationary phase of polyethylene power. Detailed spectroscopic characterization of the resolved components has resulted in the identification of four different chlorins and six different esterifying alcohols. The major esterifying alcohol is trans, trans-farnesol, but smaller amounts of geranylgeraniol, tetrahydrogeranylgeraniol, phytol, cis-9-hexadecen-1-ol and 4-undecyl-2-furanmethanol are also observed. The high information content intrinsic in the compositional analyses of the complex pigment mixtures found in green sulfur bacteria appears to provide a new probe of the mechanisms of pigment biosynthesis in these organisms.
细菌叶绿素c是绿色硫细菌嗜硫绿菌中的一种捕光色素，通过一种基于聚乙烯粉末固定相的新型高压反相色谱方法，已被分离成十几种化学性质相似的成分。对分离出的成分进行详细的光谱表征，已鉴定出四种不同的二氢卟吩和六种不同的酯化醇。主要的酯化醇是反式、反式法呢醇，但也观察到少量的香叶基香叶醇、四氢香叶基香叶醇、叶绿醇、顺式-9-十六碳烯-1-醇和4-十一烷基-2-呋喃甲醇。绿色硫细菌中复杂色素混合物的成分分析所固有的高信息含量，似乎为研究这些生物体中色素生物合成机制提供了一种新的探针。
