Department of Biophysics, Huygens Laboratory, University of Leiden, P.O. Box 9504, 2300 RA, Leiden, The Netherlands.
Photosynth Res. 1993 Feb;35(2):159-69. doi: 10.1007/BF00014746.
The chlorosomal bacteriochlorophyll (BChl) composition of the green sulfur bacteria Chlorobium vibrioforme and Chlorobium phaeovibrioides was investigated by means of normal-phase high-performance liquid chromatography. From both species a number of homologues was isolated, which were identified by absorption and (252)Cf-plasma desorption mass spectroscopy. Besides BChl d, C. vibrioforme contained a significant amount of BChl c, which may provide an explanation for the previous observation of at least two spectrally different pools of BChl in the chlorosomes of green sulfur bacteria (Otte et al. 1991). C. phaeovibrioides contained various homologues of BChl e only. Absorption spectra in acetone of BChl c, d and e, as well as bacteriopheophytin e are presented. No systematic differences were found for the various homologues of each pigment. In addition to farnesol, the mass spectra revealed the presence of various minor esterifying alcohols in both species, including phytol, oleol, cetol and 4-undecyl-2-furanmethanol, as well as an alcohol of low molecular mass, which is tentatively assumed to be decenol.
采用正相高效液相色谱法研究了绿硫细菌绿杆菌（Chlorobium vibrioforme）和绿杆菌（Chlorobium phaeovibrioides）的类囊体细菌叶绿素（BChl）组成。从这两个物种中分离出了许多同系物，通过吸收光谱和（252）Cf 等离子体解吸质谱对其进行了鉴定。除了 BChl d 之外，绿杆菌还含有大量的 BChl c，这可能为先前观察到的绿硫细菌类囊体中至少存在两种光谱上不同的 BChl 池提供了一个解释（Otte 等人，1991）。绿杆菌只含有各种 BChl e 的同系物。本文呈现了 BChl c、d 和 e 以及细菌叶绿素 e 的丙酮吸收光谱。各种同系物之间没有发现系统差异。除了法呢醇外，质谱还揭示了这两个物种中存在各种少量酯化醇，包括植醇、油醇、十六醇和 4-十一烷基-2-呋喃甲醇，以及一种低分子量醇，暂定为癸醇。
