Alvarez R, Luxoro M, Nassar-Gentina V, Szklarz G
Q J Exp Physiol Cogn Med Sci. 1980 Jul;65(3):199-205. doi: 10.1113/expphysiol.1980.sp002506.
Single giant muscle fibres from Megabalamus psittacus was microinjected with ATP-32P or internally perfused with a solution containing 32P-PArg in an attempt to demonstrate, in the absence of inhibitiors, ATP utilization during mechanical activity with negative results. The rate constant of transfer of 32P from microinjected 32P-PArg to endogenous ATP was studied at two different temperatures, obtaining a Q10 of 1.08 +/- .012. It is concluded that the rate limiting step in the process of transference of phosphate in the resting muscle from balanus is the intracellular diffusion of PArg and not the rate at which the equivalent of Lohman's reaction in invertebrates proceeds.
对来自鹦鹉巨肌的单个巨大肌纤维进行微注射ATP - 32P，或用含有32P - PArg的溶液进行内部灌注，试图在无抑制剂的情况下证明机械活动期间的ATP利用情况，但结果为阴性。在两个不同温度下研究了32P从微注射的32P - PArg向内源性ATP转移的速率常数，得到的Q10为1.08±0.012。得出结论，在静息肌肉中，来自藤壶的磷酸盐转移过程中的限速步骤是PArg的细胞内扩散，而不是无脊椎动物中相当于洛曼反应的速率。
